home Journal club A really important technique for metagenomic studies: stable isotope probing

A really important technique for metagenomic studies: stable isotope probing

Another really interesting microbial diversity paper in mBio.  This one is from Josh Neufeld and colleagues: Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities.

The key thing they did is summarized in their abstract:

We incubated samples from three disparate Canadian soils (tundra, temperate rainforest, and agricultural) with five native carbon (12C) or stable-isotope-labeled (13C) carbohydrates (glucose, cellobiose, xylose, arabinose, and cellulose). Indicator species analysis revealed high specificity and fidelity for many uncultured and unclassified bacterial taxa in the heavy DNA for all soils and substrates.

And then they sequence metagenomes from the samples and use the labelled carbon to separate out the DNA from active cells versus from inactive cells.  It is a method that has been used a few times in prior studies and this is one of the better examples I have seen.  Definitely worth a read for those thinking about going beyond just sequencing and learning about function in communities.

stable isotope probing

%d bloggers like this: