Wanted – recommended service providers for microbial sequencing (genomes, rRNA, metagenomes, etc)

In January 2014 I wrote this post about “microbiome” sequencing services. I have gotten many outside requests for the following information — what places (companies, Universities, government agencies, etc) provide contract services for rRNA PCR and sequencing? Source: Request — Information on Places that do rRNA sequencing as a service — microBEnet: the microbiology of the …

Method of interest: precision sequencing of near full 16S rRNA genes on a MiSeq (from @Cath_B & @koadman)  

Definitely worth checking this one (from Burke and Darling) out if you do microbial diversity studies … Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision. Results We describe …

New paper of interest on “Phylotags” – High-resolution phylogenetic microbial community profiling

This seems like it could be of interest to microbial community researchers: The ISME Journal – High-resolution phylogenetic microbial community profiling Abstract (with bolding by me) Over the past decade, high-throughput short-read 16S rRNA gene amplicon sequencing has eclipsed clone-dependent long-read Sanger sequencing for microbial community profiling. The transition to new technologies has provided more …

Wrap up of talk by Norm Pace on “Metagenomics and the Tree of Life”

Norm Pace gave a talk at UC Davis yesterday on “Metagenomics and the Tree of Life”. I and a few other people posted live Tweets from the talk which I have compiled together via the Storify system.  This “Storify” is embedded below. In addition, Lisa Cohen, who was at the talk posted her notes which …

Special Seminar at #UCDavis: Dr. Norman Pace on Metagenomics and the Tree of Life

Special Seminar Dr. Norman Pace Distinguished Professor Department of Molecular, Cellular and Developmental Biology University of Colorado, Boulder Metagenomics and the Tree of Life Tuesday, October 6th, 2015 1.30 pm Genome and Biomedical Science Facility (GBSF) 1005 Host: Jonathan Eisen (jaeisen@ucdavis.edu) For more about Pace’s work see http://pacelab.colorado.edu

High throughput full length 16S sequencing

Microbial ecology has benefited enormously from the development of high throughput sequencing technologies, driving the discovery of massive diversity in environments from the ocean to the human body.  Where sequencing of less than one hundred 16S rRNA genes from several samples used to be common place with cloning and Sanger sequencing, we can now generate tens …

Another important paper: microbiome studies “strongly influenced by sample processing & PCR primers”

Another paper on how sample processing (and in this case PCR primer choice) can influence microbiome studies.  And another one that is definitely worth looking at: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice. Microbiome 2015, 3:26 doi:10.1186/s40168-015-0087-4 by Alan W. Walker, Jennifer C. Martin, …