Indexed PCR Primers Induce Template-Specific Bias in Large-Scale DNA Sequencing Studies

So I had two thoughts when I read the article title “Indexed PCR Primers Induce Template-Specific Bias in Large-Scale DNA Sequencing Studies“… #1:  Ack!  Yet another thing to worry about in the insanely complicated craziness that is 16S amplicon sequencing. #2: I probably shouldn’t be the person to blog about this because I’ve honestly never …

microBEnet journal club: MetaBAT for reconstructing single genomes from complex microbial communities

    There is an interesting paper out a few days ago in PeerJ: MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities. By Dongwan D. Kang, Jeff Froula, Rob Egan, Zhong Wang​.  The key to what they do in the paper is summarized in Figure 1:   The legend is below: There are three preprocessing steps before MetaBAT is …

Best practices for sample processing and storage prior to microbiome DNA analysis freeze? buffer? process?

So – we have been having a running discussion with people in my lab about one key issue in microbiome studies – how does one store samples prior to doing DNA extractions and does it matter?  As background for those who do not do this kind of work – the general principle behind DNA based …

Genomic data sharing via BitTorrent, part 2 – BioTorrents to BitTorious

As someone who uses sequence data for most of my research,  I am continually happy with the fact that sequencing continues to get cheaper and easier and faster and bigger and better and more and more and more.  Along with such continued advances, sharing the data produced by such sequencing has become somewhat challenging at …

Healthcare Design discusses design issues relating to hospital acquired infections

Just a quick post here pointing people to an article of possible interest: Healthcare-associated Infections Keep Industry On High Alert.  In the article Sara Marberry discusses some issues relating to microbes and the built environment in hospital design.  Among the topics covered are hydrogen peroxide vapor systems, UV irradiation devices, and copper as possible antimicrobials.  Also …

Open Source Building Science Sensors

This is just a quick post to introduce some early work products resulting from a really exciting project my team has been working on: the Open Source Building Science Sensors (OSBSS) Project (funded by the Sloan Foundation). The goal of OSBSS is to to design and develop a network of inexpensive, open source devices based on …

Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS!

The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress. So far, the 16S rRNA V4 region forward primer (designated 515f) has been paired with five different reverse primers (806r, 926r, 967r, 1048r, and 1391r) to amplify ribosomal RNA from bacteria, Archaea, and …

Announcing the scikit-bio cookbook “recipe-of-the-week” series

scikit-bio is a library for building bioinformatics tools and workflows in Python. It’s already a core dependency of QIIME, is extensively used in An Introduction to Applied Bioinformatics, and was the subject of my talk at SciPy 2014 (video) this past July. One of our focuses with scikit-bio is to make its functionality very accessible …